The main goal of this project is to develop and apply an environmental DNA (eDNA) protocol for the detection and monitoring of declining Irish Arctic char (<i>Salvelinus alpinus</i>). This project has seen the participation of several students, academics and researchers since its first iteration in 2015 and it has recently resulted in a peer-reviewed publication on an international journal dedicated to the emerging application of environmental DNA (https://onlinelibrary.wiley.com/doi/full/10.1002/edn3.60). Financial support for this project has been received from the Department of Agriculture, Forestry and the Marine (DAFM) and the Genetic Resources Grant Aid Scheme (GRGAS 15/GR/15. 16/GR/12).